Recombinant Mouse Carboxypeptidase B1/CPB1 Protein (His Tag)

SKU: PKSM040708

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: 0910001A18Rik;1810063F02Rik;2210008M23Rik;AI504870

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: HEK293 Cells

Application: Enzyme

Fusion tag: C-His

UNIProt ID: B2RS76

Accession: NP_083982.1

Background: Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

Concentration:

Activity: Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg. The specific activity is > 5, 000 pmoles/min/μg.

Sequence: Met 1-Tyr 415

Purity: > 94 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20mM MES, 150mM NaCl, pH 6.5
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 47.5 kDa

ObservedMW: 43-45 kDa