Recombinant Mouse Carbonic Anhydrase 4/CA4 Protein (His Tag)

SKU: PKSM040721

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: CA-IV;CA4;CAIV;Car4;Carbonate dehydratase IV;EC4.2.1.1;RP17;carbonic anhydrase 4;carbonic anhydrase IVRP17;carbonic dehydratase IV;retinitis pigmentosa 17

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: HEK293 Cells

Application: Enzyme

Fusion tag: C-His

UNIProt ID: Q64444

Accession: NP_031633.1

Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.

Concentration:

Activity: Measured by its esterase activity. The specific activity is > 10 pmoles/min/μg.

Sequence: Met 1-Ser 277

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 31.0 kDa

ObservedMW: 31 kDa