Recombinant Mouse AFP Protein (His Tag)

SKU: PDEM100237

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:AFP

Target Synonym:AFPD;Afp;Alpha fetoglobulin;Alpha fetoprotein;Alpha fetoprotein precursor;Alpha-1-fetoprotein;Alpha-fetoglobulin;Alpha-fetoprotein;FETA;HPAFP;Hereditary persistence of;Hereditary persistence of alpha fetoprotein;alpha-fetoprotein;included

Research Areas:

Conjugation:

Target Species:Mouse

Expression Host:E.coli

Application:Immunogen

Fusion tag:N-His

UNIProt ID:P02772

Accession:P02772-1

Background:This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Concentration:

Activity:Not validated for activity

Sequence:Tyr273-Val605

Purity: > 85% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:25.9 kDa

ObservedMW:28.2 kDa