Recombinant MASH1 Monoclonal Antibody

SKU: AN301998L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:MASH1

Target Synonym:ASH;ASCL;HASH;MASH;bHLHa;ASCL1;ASH1;HASH1;MASH1;bHLHa46;Achaete scute complex homolog 1;Achaete scute complex homolog like 1;Achaete scute complex homologue 1;Achaete scute complex homologue like 1;Achaete scute complex like 1;Achaete scute family bHLH transcription factor 1;Achaete scute protein;Achaete-scute homolog 1;Ascl 1;Ash 1;ASH-1;Class A basic helix-loop-helix protein 46;Hash 1;Mammalian achaete scute homolog 1;Mammalian achaete scute homologue 1;Mash 1

Research Areas:Epigenetics and Nuclear Signaling;Neuroscience

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;

Application:WB

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A718

UNIProt ID:P50553

Accession:

Background:MASH1, also known as achaete-scute homolog 1 (ASCL1), is a basic helix-loop-helix (BHLH) transcription factor which plays an essential role in the differentiation of neuroendocrine cells and neural tissues. MASH1 directly binds the E-box motif (5'-CANNTG-3') on promoters, with dimerization with other BHLH proteins required for efficient DNA binding. Acting as a pioneer transcription factor, MASH1 also accesses closed chromatin, allowing other factors to promote transcription of neuronal genes and activate neural pathways. Research studies have shown that knockdown of the MASH1 gene leads to inhibition of growth and induction of apoptosis in SCLC cells in vitro. Additionally, MASH1 is overexpressed in both classic SCLC as well as NSCLC with neuroendocrine features, suggesting its role in the pathogenesis of those malignancies. MASH1 plays a crucial role in promoting SCLC carcinogenesis by upregulating the expression of DLL3, a nonfunctioning Notch ligand, leading to inhibition of the Notch signaling pathway.

Concentration:1 mg/mL

Immunogen:Peptide. This information is proprietary to PTMab

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:1000

Calculated MW:25 kDa

ObservedMW:30 kDa