Recombinant Macaca fascicularis Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1)

SKU: Q60HC8

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Neuroscience

Uniprot ID: Q60HC8

Gene Names: UCHL1

Alternative Name(s): (UCH-L1)(Ubiquitin thioesterase L1)

Abbreviation: Recombinant Cynomolgus monkey UCHL1 protein

Organism: Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Source: E.coli

Expression Region: 1-220aa

Protein Length: Full Length

Tag Info: N-terminal 6xHis-tagged

Target Protein Sequence: MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEDSLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEGTLLQDAAKVCREFTEREQGEVRFSAVALC

MW: 28.6 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Reference: "Localization of ubiquitin carboxyl-terminal hydrolase-L1 in cynomolgus monkey placentas." Sekiguchi S., Takatori A., Negishi T., Kwon J., Kokubo T., Ishii Y., Kyuwa S., Yoshikawa Y. Placenta 26: 99-103(2005)

Function: