Recombinant Human THEM2/ACOT13 Protein (His Tag)

SKU: PKSH032042

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: THEM2;ACOT13

Target Synonym: ACOT13;Acyl-CoA Thioesterase 13;Acyl-Coenzyme A Thioesterase 13;THEM2;Thioesterase Superfamily Member 2

Research Areas:

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: Q9NPJ3

Accession: Q9NPJ3

Background: Acyl-coenzyme A thioesterase 13, also known as Thioesterase superfamily member 2, ACOT13, THEM2 and PNAS-27, is a member of the thioesterase PaaI family. Acyl-CoA thioesterases catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. THEM2 is a cytoplasmic protein and exsis in a homotetramer. THEM2 has been identified as an interacting protein of phosphatidylcholine transfer protein. THEM2 also regulates hepatic lipid and glucose metabolism.

Concentration:

Activity: Not validated for activity

Sequence: Thr 2-Asn140

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 8% Sucrose, 100mM NaCl, 0.05% Tween 80, pH7.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 15.9 kDa

ObservedMW: 15 kDa