Recombinant Human TFF2 protein

SKU: CD00833

Size:5 ug

Background:Trefoil factor 2 encoded by the TFF2 gene in humans, belongs to the trefoil factor family that consists of three members named TFF1, TFF2 and TFF3. They are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. The TFFs are stable secretory proteins expressed highly in the gastrointestinal tract (gastric mucosa). TFF2 can inhibit gastrointestinal motility and gastric acid secretion. Additionally, it functions as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.

Research_area:Others

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Trefoil Factor 2

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 1 ng/ml, corresponding to a specific activity of > 1. × 15 IU/mg.

Synonyms:TFF2, SML1, SP,Human

Endotoxin:Less than 1 EU/μg of rHuTFF2 as determined by LAL method.

Storage:-20ºC

Sequence:EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKS VEDCHY

uniprot accession: Q343

Molecular weight:Approximately 12. kDa, a single non-glycosylated polypeptide chain containing 16 amino acids, which includes a 4-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB, pH 7.4, 13 mM NaCl.