Recombinant Human SPHK1/Sphingosine Kinase 1 Protein

SKU: PKSH030315

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: SPHK;SPHK1;SPK

Research Areas: Signal Transduction,Cancer,Metabolism

Conjugation:

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application: Enzyme

Fusion tag: None

UNIProt ID: Q9NYA1

Accession: Q9NYA1-1

Background: SPHK1; also known as sphingosine Kinase 1; catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation; survival; migration; cytoskeletal organization; and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory; antiapoptotic; and immune processes.

Concentration:

Activity: The specific activity was determined to be 1028 nmol/min/mg using Sphingosine Kinase Substrate as substrate.

Sequence: Met 1-Leu 384

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 42.7 kDa

ObservedMW: 46 kDa