Recombinant Human SPARC protein (His Tag)

SKU: CD00887

Size:10 ug

Background:Secreted protein acidic and rich in cysteine (SPARC), also named as osteonectin or BM-40, is an acronym for “secreted protein, acidic and rich in cysteine”. It is encoded by the SPARC gene in humans. The protein is a glycoprotein of 40 kDa, (303 amino acid residues) and consists of 17 a.a. signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs. SPARC is the founding member of a family of secreted matricellular proteins with similar domain structure. It is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. SPARC is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion.

Research_area:Others

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Hu Sparc

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3. μg/mL, corresponding to a specific activity of > 333 IU/mg.

Synonyms:SPARC, BM-4, OI17, ON, ONT, Human

Endotoxin:Less than 1 EU/μg of rHuSPARC, His as determined by LAL method.

Storage:-20ºC

Sequence:MSYYHHHHHH DYDIPTTENL YFQGAMGSAP QQEALPDETE VVEETVAEVT EVSVGANPVQ VEVGEFDDGA EETEEEVVAE NPCQNHHCKH GKVCELDENN TPMCVCQDPT SCPAPIGEFE KVCSNDNKTF DSSCHFFATK CTLEGTKKGH KLHLDYIGPC KYIPPCLDSE LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT EKQKLRVKKI HENEKRLEAG DHPVELLARD FE

uniprot accession:P9486;Q9BQ16; Q92563

Molecular weight:Approximately 36.1 kDa, a single non-glycosylated polypeptide chain containing 314 amino acids, with expression vector sequence (containing 6 × His tag).

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.