Recombinant Human SIGLEC2/CD22 Protein (His Tag)

SKU: PDMH100369

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:SIGLEC2;CD22

Target Synonym:B-cell receptor CD22;B-lymphocyte cell adhesion molecule;BL-CAM;CD22;CD22 antigenMGC130020;CD22 molecule;SIGLEC-2;SIGLEC2FLJ22814;Siglec-2;T-cell surface antigen Leu-14;sialic acid binding Ig-like lectin 2

Research Areas:Cancer;immunology;Stem cells

Conjugation:

Target Species:Human

Expression Host:HEK293 Cells

Application:

Fusion tag:C-His

UNIProt ID:P20273

Accession:P20273

Background:Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins, one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Concentration:

Activity:Not validated for activity

Sequence:Asp20-Arg687

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method.

Calculated MW:76.2 kDa

ObservedMW:100-140 kDa