Recombinant Human sFasR/TNFRSF6 protein

SKU: CD00923

Size:5 ug

Background:Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human soluble Fas Receptor/TNFRSF6

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 1-15 μg/ml in the presence of 2 ng/ml of rHuFas Ligand.

Synonyms:sFasR/TNFRSF6,ALPS1A, APO-1, APT1, CD951, FASTM, TNFRSF6, FAS, Human

Endotoxin:Less than 1 EU/μg of rHusFasR/TNFRSF6 as determined by LAL method.

Storage:-20ºC

Sequence:RLSSKSVNAQ VTDINSKGLE LRKTVTTVET QNLEGLHHDG QFCHKPCPPG ERKARDCTVN GDEPDCVPCQ EGKEYTDKAH FSSKCRRCRL CDEGHGLEVE INCTRTQNTK CRCKPNFFCN STVCEHCDPC TKCEHGIIKE CTLTSNTKCK EEGSRSN

uniprot accession:P25445

Molecular weight:Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.