Recombinant Human SDF-1 Alpha/CXCL12 Alpha protein

SKU: CD00953

Size:2 ug

Background:CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the C-terminal tail. And all SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to the canonical sequence SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Stromal-Cell Derived Factor-1 alpha/CXCL12α

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 2-8 ng/ml.

Synonyms:SDF-1α/CXCL12α, IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1，Human

Endotoxin:Less than 1 EU/μg of rHuSDF-1α/CXCL12α as determined by LAL method.

Storage:-20ºC

Sequence:KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

uniprot accession: P4861

Molecular weight:Approximately 8. kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB pH 7., 13 mM NaCl.