Recombinant Human R-Spondin 1/RSPO1 Protein (His Tag)

SKU: PKSH033007

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: R-Spondin 1;RSPO1

Target Synonym: CRISTIN3;FLJ40906;R-spondin;R-spondin1;RP11-566C13.1;RP23-325M14.2;RSPO Rspo1;RSPO1;Roof plate-specific spondin-1;Rspondin

Research Areas: Developmental Biology;Stem cells

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: Cell culture

Fusion tag: C-His

UNIProt ID: Q2MKA7

Accession: Q2MKA7

Background: RSPO1 is a secreted protein,containing 2 FU(furin-like) repeats and 1 TSP type-1 domain and belonging to the R-spondin family. RSPO1 is required for the early development of gonads, regardless of sex. It has been found in mice only eleven days after fertilization. To induce cell proliferation, it acts synergistically with WNT4. They help stabilize β catenin, which activates downstream targets. RSPO1 is necessary in female sex development. It augments the WNT/β catenin pathway to oppose male sex development. In critical gonadal stages, between six and nine weeks after fertilization, the ovaries upregulate it while the testes downregulate it. RSPO1 can potentially aid in the treatment of mucositis, which is characterized by inflammation of the oral cavity. This unfortunate condition often accompanies chemotherapy and radiation in cancer patients with head and neck tumors.

Concentration:

Activity: Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED₅₀ for this effect is 1-10ng/ml.

Sequence: Ser21-Ala263

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.

Calculated MW: 27.8 kDa

ObservedMW: 40 kDa