Recombinant Human PUMA/BBC3 protein (His Tag)

SKU: PDEH100697

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:PUMA;BBC3

Target Synonym:BBC 3;BBC3_HUMAN;BCL 2 binding component 3;BCL2 binding component 3;Bbc3;Bcl-2-binding component 3;JFY 1;JFY-1;JFY1;PUMA alpha;PUMA/JFY1;p53 Upregulated Modulator of Apoptosis;p53 up regulated modulator of apoptosis;p53 up-regulated modulator of apoptosis

Research Areas:Cell Biology,Signal Transduction,Cancer,Metabolism

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:Immunogen

Fusion tag:N-His

UNIProt ID:Q9BXH1

Accession:Q9BXH1-1

Background:Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis . Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.

Concentration:

Activity:Not validated for activity

Sequence:Met1-Asn193

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4., 5% trehalose, 5% mannitol, 0.01% Tween 80.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:20.1 kDa

ObservedMW:20-24 kDa