Recombinant Human PTP1B/PTPN1 Protein (His Tag)

SKU: PKSH030416

Size: 100μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: PTP1B;PTPN1

Research Areas: Signal Transduction;Cardiovascular;

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Enzyme

Fusion tag: N-His

UNIProt ID: P18031

Accession: NP_002818.1

Background: PTP1B, also known as PTPN1, belongs to the protein-tyrosine phosphatase (PTP) family. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTP1B contains 1 tyrosine-protein phosphatase domain and is expressed in many tissues. PTP1B is localized to the cytoplasmic face of the endoplasmic reticulum. PTP1B was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of PTP1B in cell growth control, and cell response to IFN stimulation.

Concentration:

Activity: Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor (aa988-998) phosphopeptide substrate, R&D Systems, Catalog # ES006. The specific activity is > 15 nmoles/min/μg.

Sequence: Glu 2-Asn 321

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of PBS, pH 7.4

Reconstitution: Not Applicable

Endotoxin: Please contact us for more information.

Calculated MW: 38 kDa

ObservedMW: 38 kDa