Recombinant Human PTH1-84 (15N Stable Isotope Labeled)

SKU: CD01032

Size:10 ug

Background:Parathyroid hormone (PTH) is a single polypeptide of 84 amino acids. It is a critical hormone in the regulation of Ca2+ homeostasis and secreted by the parathyroid glands, which promote release of calcium from bone to extracellular fluid by activating osteoblasts and inhibiting osteoclasts, indirectly promote increased intestinal absorption of calcium, and promote renal tubular reabsorption of calcium and increased renal excretion of phosphates. It is a major regulator of bone metabolism. Secretion of parathyroid hormone increases when the level of calcium in the extracellular fluid is low.

Research_area:Others

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Parathyroid Hormone 1-84, 15N Stable Isotope Labeled

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by its ability to induce cAMP accumulation in Mouse MC3T3E1 cells is less than 5 ng/ml, corresponding to a specific activity of > 2. × 14 IU/mg.

Synonyms:PTH,FIH1, PTH1, Human

Endotoxin:Less than 1 EU/μg of rHuPTH1-84, 15N as determined by LAL method.

Storage:-20ºC

Sequence:SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ

uniprot accession:P127

Molecular weight:Approximately 9551 Da, a single non-glycosylated polypeptide chain containing 84 amino acids. 15N stable isotope labeled.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.