Recombinant Human PRCP Protein (His Tag)

SKU: PKSH032721

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: PRCP

Target Synonym: Angiotensinase C;Lysosomal Carboxypeptidase C;Lysosomal Pro-X Carboxypeptidase;PCP;PRCP;Proline Carboxypeptidase;Prolylcarboxypeptidase

Research Areas: Cell biology;Cardiovascular

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P42785

Accession: P42785

Background: Lysosomal Pro-X Carboxypeptidase (PRCP) belongs to the peptidase S28 family. PRCP is detected in many tissues, with highest levels observed in placenta, lung, and liver. It is also present in the heart, brain, pancreas, and kidney. PRCP exists as a homodimer. PRCP cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. PRCP has been shown to be an activator of the cell matrix-associated prekallikrein.

Concentration:

Activity: Not validated for activity

Sequence: Leu22-His496

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM NaAc-HAc, 150mM NaCl, 10% Glycerol, pH4.5.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 54.5 kDa

ObservedMW: 60-90 kDa