Recombinant Human OCLN Protein(GST Tag)

SKU: PDEH101112

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:OCLN

Target Synonym:PPP1R;PTORCH;OCLN;BLCPMG;PPP1R115;PTORCH1;occludin

Research Areas:Cell Biology;Cancer;Neuroscience

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-GST

UNIProt ID:Q16625

Accession:Q16625

Background:OCLN (Occludin) is a Protein Coding gene. This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. It belongs to the ELL/occludin family. OCLN is an important component of the tight junction complex, providing apical intercellular connections between adjacent cells in endothelial and epithelial tissue. OCLN, an integral tight junction protein, is one of the key factors for HCV entry into cells. OCLN, a key receptor for HCV, is a candidate target of miR-122; the most abundant hepatic micro RNA. Over-expression of miR-122 can decrease HCV entry into hepatocytes through down-regulation of OCLN. Diseases associated with OCLN include Pseudo-Torch Syndrome 1 and Torch Syndrome.

Concentration:

Activity:Not validated for activity

Sequence:Arg269-Thr522

Purity:> 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method

Calculated MW:53.8 kDa

ObservedMW:58 kDa