Recombinant Human NT-4 protein

SKU: CD01192

Size:2 ug

Background:NT-4 also named as NT-5 is a neuronal and epithelial grow factor belongs to the NGF-beta family. The NT-4 precursor is consisted of a 24 a.a. signal peptide, a 56 a.a. propertied and 130 a.a. NT-4. The mature protein has six Cys amino acid residues and has the relative structure with NT-3, BDNF (sharing about 48 % - 52 % sequence identity). Additionally, it shares 91 % and 95 % a.a. sequence identity with mouse and rat NT-4. NT-4 is mainly expressed in prostate and has low level thymus, placenta, and skeletal muscle. It can binding with the LNGFR and trkB receptors and plays a crucial role in the regulation of survival and the maintenance of peripheral sensory sympathetic neurons. Defect of NT-4 may cause primary open angle glaucoma type 1O.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Neurotrophin-4

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 5 ng/ml, corresponding to a specific activity of > 2. × 14 IU/mg.

Synonyms:NTF4G,LC1, GLC1O, NT-4, NT-4/5, NT-5, NT4, NT5, NTF5, Human

Endotoxin:Less than 1 EU/μg of rHuNT-4 as determined by LAL method.

Storage:-20ºC

Sequence:MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A

uniprot accession:P3413

Molecular weight:Approximately 28.1 kDa, a noncovalently linked homodimer of two 14. kDa polypeptide monomers (262 total amino acid residues).

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 5.5.