Recombinant Human NRG1- Beta 2 protein

SKU: CD01197

Size:10 ug

Background:Neuregulin 1 belongs to a family of structurally related polypeptide growth factors and is produced in numerous isoforms by alternative splicing, which allows it to perform a wide variety of functions. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They all have the Ig and EGF-like domain, and can bind to ErbB3 and ErbB4 receptor tyrosin kinases. This binding induces ErbB3 and ErbB4 heterodimerization with ErbB2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation. NRG1 isoforms have functions of inducing the growth and differentiation of epithelial, neuronal, glial, and other types of cells.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Neuregulin 1-beta2 EGF-like domain

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 5 ng/ml, corresponding to a specific activity of > 2. × 14 IU/mg.

Synonyms:NRG1-β2, Human

Endotoxin:Less than 1 EU/μg of rHuNRG1-β2 as determined by LAL method.

Storage:-20ºC

Sequence:SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKAEELY Q

uniprot accession:Q2297

Molecular weight:Approximately 7. kDa, a single non-glycosylated polypeptide chain containing 61 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered solution in 2 mM PB, pH 7., containing .5 % HAS and 2 % mannitol.