Recombinant Human N-Glycosylase/OGG1 Protein

SKU: PKSH032809

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: N-Glycosylase;OGG1

Target Synonym: 8-Oxoguanine DNA Glycosylase;AP Lyase;DNA-(Apurinic or Apyrimidinic Site) Lyase;MMH;MUTM;N-Glycosylase/DNA Lyase;OGG1;OGH1

Research Areas: Cancer;epigenetics and nuclear signal

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: None

UNIProt ID: O15527

Accession: AAH00657.1

Background: Human N-Glycosylase/DNA Lyase(OOG1) is a DNA repair enzyme, which belongs to the type-1 OGG1 family. OOG1 incises DNA at 8-oxoG residues, and excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damage DNA. It has a β-lyase activity that nicks DNA 3’ to the lesion. OOG1 together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells. The OGG1 gene mutations may be caused Renal cell carcinoma.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Gly345

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM EDTA, pH 8.5.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 38.8 kDa

ObservedMW: 38 kDa