Recombinant Human/Mouse FGF-8b/FGF8B Protein

SKU: PKSH033805

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: FGF-8b;FGF8B

Target Synonym: AIGF;Androgen-induced growth factor;FGF-8B;Fibroblast growth factor 8;HBGF-8;Heparin-binding growth factor 8

Research Areas:

Conjugation:

Target Species: Human/Mouse

Expression Host: E.coli

Application: Cell culture

Fusion tag: None

UNIProt ID: P55075;P37237

Accession: P55075-3/P37237-2

Background: Fibroblast growth factor 8 (FGF-8) is a member of the fibroblast growth factor family. It is discovered as a growth factor essential for the androgen--dependent growth of mouse mammary carcinoma cells. Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial--mesenchymal transitions. It plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. It is required for normal brain, eye, ear, limb development during embryogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.

Concentration:

Activity: Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED₅₀ for this effect is 21.87 ng/ml.

Sequence: Gln23-Arg215

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB,300mM NaCl,2% Sucrose,0.02% Tween 80,pH7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.

Calculated MW: 22.5 kDa

ObservedMW: 23 kDa