Recombinant Human MIP-1 Alpha/CCL3 protein

SKU: CD01282

Size:5 ug

Background:CCL3, also known as macrophage inflammatory protein-1α (MIP-1α), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. Studies showed CCL3 can bind to CCR1, CCR4 and CCR5. MIP-1α can induce a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Recombinant human MIP-1α is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines and it shares approximately 73 %, 74 % and 92 % amino acid sequence homology with the Mouse, rat and rhesus macaque protein.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Macrophage Inflammatory Protein-1 alpha/CCL3

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.-1 ng/ml.

Synonyms:MIP-1α/CCL3, GS19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3,Human

Endotoxin:Less than 1 EU/ug of rHuMIP-1α/CCL3 as determined by LAL method.

Storage:-20ºC

Sequence:ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA

uniprot accession:P1147

Molecular weight:Approximately 7.8 kDa protein containing 7 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB, pH 7.4, 1 mM NaCl.