Recombinant Human MAP2 Protein(Trx Tag)

SKU: PDEH100647

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:MAP2

Target Synonym:Microtubule associated protein;Microtubule-associated protein;MTAP;DKFZp686I;MAP;MAP2;MAP2A;MAP2B;MAP2C;DKFZp686I2148;MAP 2;MAP dendrite specific;MAP-2;Microtubule associated protein 2;Microtubule-associated protein 2;MTAP2

Research Areas:Neuroscience;Signal Transduction;Stem Cells

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-Trx

UNIProt ID:P11137

Accession:P11137

Background:METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a protein that belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.

Concentration:

Activity:Not validated for activity

Sequence:Met1-Asp230

Purity:> 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method

Calculated MW:45.3 kDa

ObservedMW:50 kDa