Recombinant Human LCAT Protein (His Tag)

SKU: PKSH032685

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: LCAT

Target Synonym: Phosphatidylcholine-sterol acyltransferase;Phospholipid-cholesterol acyltransferase and LACT;also named Lecithin-cholesterol acyltransferase;is an extracellular cholesterol esterifying enzyme which belongs to the AB hydrolase superfamily.

Research Areas: Signal Transduction;Cardiovascular;Cancer;metabolism;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P04180

Accession: P04180

Background: Lipase family. The gene encoding this protein is expressed mainly in brain, liver and testes,followed by secreting into plasma and cerebral spinal fluid. The esterification of cholesterol is required for cholesterol transport. LCAT is a central enzyme in the extracellular metabolism of plasma lipoproteins. Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD) and a cause of fish-eye disease (FED).

Concentration:

Activity: Not validated for activity

Sequence: Phe25-Glu440

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 4mM HCl.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 48.1 kDa

ObservedMW: 66 kDa