Recombinant Human JAM-A/F11R Protein (Fc Tag)

SKU: PKSH031772

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: CD321;F11R;JAM-1;JAM-A;JAM1;JAMA;JCAM;Junctional Adhesion Molecule 1;Junctional Adhesion Molecule A;KAT;PAM-1;Platelet Adhesion Molecule 1;Platelet F11 Receptor

Research Areas: Signal Transduction;Cardiovascular;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: Cell culture

Fusion tag: C-hFc

UNIProt ID: Q6FIB4

Accession: NP_058642.1

Background: Junctional adhesion molecule-A (JAM-A); also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321); is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells; as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1; involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily; JAM-A protein involves in platelet adhesion; secretion and aggregation; and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition; it may be a potential therapeutic target for breast cancer.

Concentration:

Activity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 x 10⁴cells/well are added to JAM-A-Fc coated plates (2. 5μg/mL, 100 μL/well)in the presence of 20 ng/mL PMA, approximately 30-40% will adhere after 30 minutes at 37℃.

Sequence: Met 1-Ala 242

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 50.0 kDa

ObservedMW: 61 kDa