Recombinant Human IL5RA/IL-5 Rα Protein (Fc Tag)

SKU: PKSH032652

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: IL5RA;IL-5 Rα

Target Synonym: CDw;HSIL5R;IL5RA;CD125;CDw125;HSIL5R3;IL5R;IL-5 receptor subunit alpha;IL-5R subunit alpha;IL-5RA;IL-5R-alpha;Interleukin-5 receptor subunit alpha; CDw125;IL-5RαCD125 antigen;IL5R;Interleukin 5 Receptor alpha;Interleukin 5 Receptor alpha chain;Interleukin 5 receptor alpha subunit;Interleukin 5 receptor subunit alpha;Interleukin 5 receptor type 3;MGC26560

Research Areas: immunology

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-Fc

UNIProt ID: Q01344

Accession: Q01344

Background: Interleukin-5 Receptor alpha (IL-5Rα; CD125) is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology. Mature human IL-5 Rα consists of a 322 aa extracellular domain (ECD) with a WSxWS motif and a four cysteine motif; a 20 aa transmembrane segment; and a 58 aa cytoplasmic domain. Within the ECD; human IL-5Rα shares 71% aa sequence identity with mouse and rat IL-5 Rα. Alternate splicing of human IL-5 Rα generates soluble secreted forms which function as IL-5 antagonists. The high affinity receptor for IL-5 is a complex that consists of the ligand binding IL-5 Rα and the transmembrane common β chain (βc/CD131) which is shared with the receptor complexes for IL-3 and GMCSF. IL-5 Rα binds IL-5 at low affinity and then associates with preformed βc oligomers to form the signaling competent receptor complex. IL-5 stimulation of CD34+ hematopoietic progenitor cells induces the up-regulation of transmembrane IL-5Rα followed by eosinophilic differentiation and activation.

Concentration:

Activity: Not validated for activity

Sequence: Asp21-Glu335

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 62.5 kDa

ObservedMW: 70-85 kDa