Recombinant Human IL1R2/CD121b Protein

SKU: PKSH031864

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: IL1R2/CD121b

Target Synonym: IL1R;Interleukin-1 receptor type;IL-1R;IL1R2;CD121b;CDw121b;IL-1R-2;IL-1RT-2;IL-1RT2;IL1R2c;IL1RB;CD121 antigen-like family member B;IL-1 type II receptor;IL-1R-beta;Interleukin-1 receptor beta;Interleukin-1 receptor type 2;Interleukin-1 receptor type I;IL-1R type II; IL-1RII

Research Areas: Cardiovascular;immunology;Stem cells

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: None

UNIProt ID: P27930

Accession: NP_004624.1

Background: Interleukin 1 receptor; type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A); interleukin beta (IL1B); and interleukin 1 receptor; type I (IL1R1/IL1RA); and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate development and maintenance of the inflammatory responses; and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified; designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling; whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand binding portion comprised of three Ig-like domains; a single transmembrane region; and a short cytoplasmic domain; is expressed in a variety of cell types including B lymphocytes; neutrophils; monocytes; large granular leukocytes and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.

Concentration:

Activity: Immobilized human IL1R2 at 10 μg/ml (100 μl/well) can bind biotinylated human IL1B-His, The EC50 of biotinylated human IL1B-His is 0.14-0.34 μg/ml.

Sequence: Met 1-Glu 343

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 38.4 kDa

ObservedMW: 49 kDa