Recombinant Human IL-2 protein

SKU: CD01529

Size:10 ug

Background:IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL-2Rα, 75 kDa IL-2Rβ, and 64 kDa common gamma chain γc/IL-2Rγ) that are present on the cell surface in varying preformed complexes. Mature human IL-2 shares 56 % and 66 % amino acid sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Interleukin-2

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse CTLL-2 cells is less than .1 ng/ml, corresponding to a specific activity of > 1. × 17 IU/mg.

Synonyms:IL-2, IL2, TCGF, lymphokine,Human

Endotoxin:Less than 1.0 EU/μg of rHuIL-2 as determined by LAL method.

Storage:-20ºC

Sequence:APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT

uniprot accession:P6568

Molecular weight:Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 5..