Recombinant Human IGF-1 protein, (15N Stable Isotope Labeled)

SKU: CD01594

Size:10 ug

Background:The insulin-like growth factors (IGFs) belonged to the insulin gene family, are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Recombinant human IGF-1 are globular proteins containing 70 amino acids and 3 intra-molecular disulfide bonds. Mature human IGF-1 shares 94 % and 96 % a.a. sequence identity with mouse and rat IGF-1, respectively, and exhibits cross-species activity.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Insulin-like Growth Factor-1, 15N Stable Isotope Labeled

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5. × 15 IU/mg.

Synonyms:IGF-1, IGF, IGFI, IGFI, MGF,15N, Human

Endotoxin:Less than 1 EU/μg of rHuIGF-1, 15N as determined by LAL method.

Storage:-20ºC

Sequence:GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

uniprot accession:P519

Molecular weight:Approximately 7.7 kDa, a single non-glycosylated polypeptide chain containing 7 amino acids. 15N stable isotope labeled.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.2.