Recombinant Human hnRNPK protein (GST,His tag)

SKU: PDEH101052

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:hnRNPK

Target Synonym:HNRPK;Heterogeneous nuclear ribonucleoprotein K;Transformation up-regulated nuclear protein (TUNP)

Research Areas:Epigenetics and Nuclear signaling

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-GST & C-His

UNIProt ID:P61978

Accession:P61978-2

Background:One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded

Concentration:

Activity:Not validated for activity

Sequence:Met 1-Lys 219

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:49.0 kDa

ObservedMW:50 kDa