Recombinant Human HMBS Protein (His Tag)

SKU: PKSH032918

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: HMBS

Target Synonym: HMBS;Hydroxymethylbilane Synthase;PBG-D;PBGD;Porphobilinogen Deaminase;Pre-Uroporphyrinogen Synthase;UPS

Research Areas: Signal Transduction;Cell biology;metabolism;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P08397

Accession: P08397

Background: Porphobilinogen Deaminase (HMBS) is a member of the HMBS family. PBGD is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. HMBS is involved in the production of heme, which is important for all of the body's organs, although it is most abundant in the blood, bone marrow, and liver. In addition, Heme is an essential component of iron-containing proteins called hemoproteins, including hemoglobin. Defects in PBGD are the cause of acute intermittent porphyria.

Concentration:

Activity: Not validated for activity

Sequence: Ser2-His361

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, 5% mannitol, 50% Glycerol, 0.1% Tween 80, pH7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 40.5 kDa

ObservedMW: 47 kDa