Recombinant Human HIF1A Protein

SKU: PDEH100740

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:HIF1A

Target Synonym:ARNT-interacting protein;BHLHE78;Basic-helix-loop-helix-PAS protein MOP1;Class E basic helix-loop-helix protein 78;HIF-1-alpha;HIF1-alpha;Hypoxia-inducible factor 1-alpha;MOP1;Member of PAS protein 1;PAS domain-containing protein 8;PASD8;bHLHe78

Research Areas:Immunology;Cardiovascular

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:Q16665

Accession:Q16665

Background:Functions as a master transcriptional regulator of the adaptive response to hypoxia.Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia.Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease.Heterodimerizes with ARNT, heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters.

Concentration:

Activity:Not validated for activity

Sequence:Ser 576-Gln785

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4., 5% trehalose, 5% mannitol, 0.01% Tween 80.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:25.2 kDa

ObservedMW:26 kDa