Recombinant Human GPNMB Protein (aa 22-486, His Tag)

SKU: PKSH033371

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: GPNMB

Target Synonym: GPNMB;HGFIN;NMB;Osteoactivin;Transmembrane Glycoprotein HGFIN;Transmembrane Glycoprotein NMB

Research Areas: Immunology,Cancer

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: Q14956

Accession: Q14956

Background: Osteoactivin is an intracellular glycoprotein belongs to the NMB/pMEL-17 family; which is asscociated with cell endosomal/lysomal compartments. Human Osteoactivin is a 560 amino acid type I transmembrane protein; and one alternate splice form shows a 12 amino acid insert between amino acid 339-340. An additional 206 amino acid isoform shows a mutation at position 181 that results in a 26 amino acid substitution for the C-terminal 380 amino acids. Cells knowns to express Osteoactivin include fibroblast; osteoblasts; myeloid dendritic cell; melanocytes; plus fetal chondrocytes and stratum basale keratinocytes; macrophages/keratinocytes.

Concentration:

Activity: Not validated for activity

Sequence: Ala22-Pro486

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 53.1 kDa

ObservedMW: 80-120 kDa