Recombinant Human GH protein

SKU: CD01727

Size:20 ug

Background:Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is a peptide hormone that stimulates growth, cell reproduction and regeneration in humans and other animals. Growth hormone is a 191-amino acid, single-chain polypeptide that is synthesized, stored, and secreted by somatotropic cells within the lateral wings of the anterior pituitary gland. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones-GH-releasing hormone (GHRH) and somatostatin (SST) as well as by signals from the periphery-ghrelin and leptin. The human GH cDNA encodes a 217 amino acid (a.a.) residue precursor protein with a 26 a.a. putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified.

Research_area:Others

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Growth Hormone

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using rat Nb2-11 lymphoma cells is less than .1 ng/ml, corresponding to a specific activity of > 1. × 17 IU/mg.

Synonyms:GH,GH1, GH-N, GHB5, GHN, IGHD1A, IGHD1B, IGHD2, hGH-N, Human

Endotoxin:Less than 1EU/μg of rHuGH as determined by LAL method.

Storage:-20ºC

Sequence:FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS LVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F

uniprot accession:P1241

Molecular weight:Approximately 22 kDa, a single non-glycosylated polypeptide chain containing 191 amino acids

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution with 5mM NaHCO3, pH 7..