Recombinant Human Fibronectin/FN Protein

SKU: PKSH032450

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: Fibronectin;FN

Target Synonym: CIG;ED-B;FINC;FN;FN1;FNZ;Fibronectin;GFND;GFND2;LETS;MSF

Research Areas: Signal Transduction;Cardiovascular;Cancer;Developmental Biology;Stem cells

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: None

UNIProt ID: P02751

Accession: P02751

Background: Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains;fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment; is a protein of ~63 kDa containing a central cell-binding domain; a high affinity heparin-binding domain II;and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand; a CS-I site; and a VLA-5 ligand; a cell attachment domain; and virus vectors binds to a heparin binding domain II; which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors; and facilitates the gene transduction in the result.

Concentration:

Activity: Not validated for activity

Sequence: Pro1270-Ser1546&Ala1721-Thr2016

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 12.5 mM Citric acid, 1.25% Sucrose, 0.1% Tween 80, pH 5.5 .
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.

Calculated MW: 62.7 kDa

ObservedMW: 60-80 kDa