Recombinant Human FGF-7/KGF-1 protein

SKU: CD01800

Size:2 ug

Background:Human KGF-1 also known as Fibroblast growth factor 7 (FGF-7), is encoded by the FGF7 gene. KGF-1 only binds to the b splice form of the tyrosine kinase receptor, FGFR2b/KGFR. Affinity between KGF-1 and its receptor can be increased by heparin or heparin sulfate proteoglycan. FGF-10, also called keratinocyte growth factor 2 (KGF-2), shares 51 % amino acid sequence identity and similar function to KGF-1, but uses an additional receptor, FGFR2c. KGF-1 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. KGF-1 actives on keratinocytes, and exhibits mitogenic activity for epidermal cells, but essentially no activity for fibroblasts. KGF-1 has species crossactive, human KGF-1 shares 96 % and 92 % amino acid sequence identity with Mouse and rat FGF-7, respectively.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Keratinocyte Growth Factor-1/FGF-7

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1. × 15 IU/mg.

Synonyms:FGF-7/KGF-1,Fgf5b, KGF/FGF7, Kgf,FGF7, Human

Endotoxin:Less than 1 EU/μg of rHuKGF-1/FGF-7 as determined by LAL method.

Storage:-20ºC

Sequence:CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT

uniprot accession:P21781

Molecular weight:Approximately 18.9 kDa, a single, non-glycosylated polypeptide chain containing 163 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered solution in 2 mM PB, .5 M NaCl, pH 8..