Recombinant Human Deoxycytidine Kinase/DCK Protein (His &T7 Tag)

SKU: PKSH032348

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: Deoxycytidine Kinase;DCK

Target Synonym: DCK;Deoxycytidine Kinase;dCK

Research Areas: Signal Transduction;Cancer;

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-His-T7

UNIProt ID: P27707

Accession: P27707

Background: Deoxycytidine Kinase (DCK) is a member of the DCK/DGK family. DCK exists as a homodimer and is localized to the nucleus. DCK is required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG), and deoxyadenosine (dA). DCK has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. In addition, DCK is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. DCK is clinically important because of its relationship to drug resistance and sensitivity.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Leu260

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of PBS, 50% Glycerol, 1mM TCEP, pH 7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 34.0 kDa

ObservedMW: 30 kDa