Recombinant Human Coagulation Factor X/F10 Protein (Fc Tag)

SKU: PKSH033714

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: Coagulation Factor X;F10

Target Synonym: Coagulation factor 10;FX;FXA;coagulation factor X

Research Areas: Cardiovascular

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-Fc

UNIProt ID: P00742

Accession: P00742

Background: F10, also known as Coagulation factor X, belongs to the peptidase S1 family that is synthesized as a 488 amino acid (aa) with a signal peptide and a pro region (residues 1-40). Both the intrinsic and extrinsic pathways activate Factor X to Xa, which consists of light (residues 41-179) and heavy (residues 235-488) chains linked by a disulfide bond. Coagulation factor X is initially synthesized in the liver. The two chains are formed from a single-chain precursor by the excision of two Arg residues and are held together by 1 or more disulfide bonds. Forms a heterodimer with SERPINA5. F10 is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

Concentration:

Activity: Not validated for activity

Sequence: Asn32-Lys488

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 0.2mM CaCl₂, pH 5.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 78.2 kDa

ObservedMW: 80-120&25 kDa