Recombinant Human CD28/TP44 Protein (Fc Tag)

SKU: PKSH032211

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Immune Checkpoint Proteins

Abbreviation: CD28;TP44

Target Synonym: CD28;CD28 antigen;CD28 molecule;T-cell-specific surface glycoprotein CD28;TP44;Tp44

Research Areas: immunology;Stem cells

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-Fc

UNIProt ID: P10747

Accession: P10747

Background: T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals; which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival; interleukin-2 production; and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands; the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Concentration:

Activity: Not validated for activity

Sequence: Asn19-Pro152

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 42.3 kDa

ObservedMW: 60-80 kDa