Recombinant Human Carboxylesterase 1/CES1 Protein (His Tag)

SKU: PKSH032167

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: Carboxylesterase 1;CES1

Target Synonym: ACAT;Acyl-Coenzyme A:Cholesterol Acyltransferase;Brain Carboxylesterase hBr1;CE-1;Carboxylesterase 1;Cocaine Carboxylesterase;Egasyn;HMSE;Liver Carboxylesterase 1;Methylumbelliferyl-Acetate Deacetylase 1;Monocyte/Macrophage Serine Esterase;Retinyl E;hCE-1

Research Areas: Cardiovascular,Signal Transduction,Metabolism

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P23141

Accession: P23141-3

Background: Carboxylesterase 1 (CES1) is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. These enzymes have broad substrate specificity ranging from small molecule esters such as phenylester to long chain fatty acid esters and thioesters. They are major determinants of the pharmacokinetic behavior of most therapeutic agents containing an ester or amide bond. CES1 shares the serine hydrolase fold observed in other esterases. CES1 hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. CES1 participates in detoxification of drugs such as cocaine and heroin in serum and liver. It may also play a role in detoxification in the lung and/or protection of the central nervous system from ester or amide compounds.

Concentration:

Activity: Not validated for activity

Sequence: His19-Glu562

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 4.0.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 61.1 kDa

ObservedMW: 60 kDa