Recombinant Human BCAS2/DAM1 Protein (His & T7 Tag)

SKU: PKSH032111

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: BCAS2;DAM1

Target Synonym: BCAS2;Breast Carcinoma-Amplified Sequence 2;DAM1;DNA Amplified in Mammary Carcinoma 1 Protein;Pre-mRNA-Splicing Factor SPF27;Spliceosome-Associated Protein SPF 27

Research Areas: Tags & Cell Markers;epigenetics and nuclear signal

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-T7 & C-His

UNIProt ID: O75934

Accession: O75934

Background: Breast Carcinoma-Amplified Sequence 2 (BCAS2) is a member of the SPF27 family. BCAS2 is a nuclear protein and widely expressed in many rtissues. BCAS2 is identified as being overexpressed in various breast cancer cell lines. BCAS2 is a component of the spliceosome, taking part in the removal of introns from mRNA precursors. BCAS2 interacts with estrogen receptor alpha and beta, thyroid hormone receptor beta, peroxisome proliferator-activated receptor gamma. BCAS2 functions as an ER co-activator and is capable of enhancing ER-mediated transcription.

Concentration:

Activity: Not validated for activity

Sequence: Ala2-Phe225

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, 2mM DTT, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 28.6 kDa

ObservedMW: 60 kDa