Recombinant Human ApoA-I protein

SKU: CD02314

Size:20 ug

Background:Apolipoprotein A-I is produced in the liver and intestine, and secreted as the predominant constituent of nascent high-density lipoprotein (HDL) particle. ApoA-I, which is found exclusively in HDL, has a unique ability to capture and solubilize free cholesterol. This ApoA-I ability enables HDL to remove excess peripheral cholesterol and return it to the liver for recycling and excretion. This process, called reverse cholesterol transport, is thought to inhibit atherogenesis. For this reason HDL is also known as the “good cholesterol.” The therapeutic potential of ApoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of ApoA-I (called ApoA-I Milano). The availability of recombinant normal ApoA-I should facilitate further investigation into the potential usefulness of ApoA-I in preventing atherosclerotic vascular diseases. Recombinant human ApoA-I is a 28.1 kDa protein of 243 amino acid residues.

Research_area:Cardiovascular

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Apolipoprotein A-I

Biological activity:Data Not Available.

Synonyms:APOA1,ApoA-I, HPALP2, apo(a),Human

Endotoxin:Less than 0.1 EU/μg of rHuApoA-I as determined by LAL method.

Storage:-20ºC

Sequence:DEPPQSPWDR VKDLATVYVD VLKDSGRDYV SQFEGSALGK QLNLKLLDNW DSVTSTFSKL REQLGPVTQE FWDNLEKETE GLRQEMSKDL EEVKAKVQPY LDDFQKKWQE EMELYRQKVE PLRAELQEGA RQKLHELQEK LSPLGEEMRD RARAHVDALR THLAPYSDEL RQRLAARLEA LKENGGARLA EYHAKATEHL STLSEKAKPA LEDLRQGLLP VLESFKVSFL SA

uniprot accession:P2647;Q6LEJ8

Molecular weight:Approximately 28.1 kDa, a single non-glycosylated polypeptide chain containing 243 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.