Recombinant HIF1 beta Monoclonal Antibody

SKU: E-AB-81565

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Recombinant Antibodies

Abbreviation:HIF1 beta

Target Synonym:arnT;ARNT protein;ARNT;Aryl hydrocarbon receptor nuclear translocator;bHLHe2;Class E basic helix-loop-helix protein 2;Dioxin receptor;Dioxin receptor nuclear translocator;Drnt;HIF 1 beta;HIF 1beta;HIF-1-beta;HIF1-beta;HIF1B;HIF1beta;Hypoxia Inducible Factor 1;Hypoxia inducible factor 1 beta;Hypoxia-inducible factor 1-beta;nuclear translocator;Tango

Research Areas:Cancer;Cardiovascular;Epigenetics and Nuclear Signaling;Metabolism

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Mouse;Rat;Hamster

Application:WB;IHC-P

Isotype:IgG

Clonality:Rabbit Monoclonal

Clone NO.:R05-3B2

UNIProt ID:P27540

Accession:

Background:Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8).

Concentration:300 μg/mL

Immunogen:Recombinant protein of human HIF1 beta

Buffer:50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein

Purification method:Affinity Purified

Dilution:WB 1:500-1:1000;IHC 1:50-1:100

Calculated MW:87kDa

ObservedMW:87kDa