Recombinant Cynomolgus TSLP (C-6His)

SKU: EPT093

Size:10ug

Description:Recombinant Cynomolgus Thymic Stromal Lymphopoietin is produced by our E.coli expression system and the target gene encoding Tyr29-Gln159(Glu37Gln) is expressed with a 6His tag at the C-terminus.

Accession:XP_005557555.1

Molecular weight:16.2 KDa

Apparent molecular weight:16 KDa, reducing conditions

Other names:Thymic stromal lymphopoietin;Thymic stroma-derived lymphopoietin;Tslp

Storage condition:Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Purity:Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin:Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

Biological activity:Immobilized Anti-Human TSLP mAb (Cat#NC066) at 2μg/ml (100 μl/well) can bind Cynomolgus TSLP-His (Cat#CR62). The ED50 of Cynomolgus TSLP-His (Cat#CR62) is 4.48 ng/ml.

Redissolve:Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage:Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Delivery condition:The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Background:Thymic stromal lymphopoietin (TSLP) is a protein belonging to the cytokine family, contains 140 amino acids. It is known to play an important role in the maturation of T cell populations through activation of antigen presenting cells. TSLP induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c+ dendritic cells. It can induce allergic inflammation by directly activating mast cells. TSLP is produced mainly by non-hematopoietic cells such as fibroblasts, epithelial cells and different types of stromal or stromal-like cells. These cells are located in regions where TSLP activity is required.