Recombinant CPS1 Monoclonal Antibody

SKU: AN301906L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:CPS1

Target Synonym:CPS;carbamoyl-phosphate synthase;CPSASE;CPS1;CPSASE1;PHN;carbamoyl-phosphate synthase 1;carbamoyl-phosphate synthase 1;CPSASE1;PHN;Carbamoyl phosphate synthase;Carbamoyl phosphate synthase [ammonia];Carbamoyl phosphate synthase [ammonia] mitochondrial;Carbamoyl phosphate synthetase 1;Carbamoyl phosphate synthetase 1 mitochondrial;Carbamoyl phosphate synthetase I;Carbamoylphosphate synthase;Carbamoyl-phosphate synthase [ammonia];Carbamoylphosphate synthetase 1;Carbamoylphosphate synthetase I;Carbamoyl-phosphate synthetase I;CPS 1;CPSase 1;CPSase I;CPSM;mitochondrial;MS738

Research Areas:Tags & Cell Markers;Signal Transduction;Metabolism

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;IHC

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A622

UNIProt ID:P31327

Accession:

Background:Carbamoyl phosphate synthetase 1 (CPS1), a rate-limiting enzyme in the urea cycle, catalyzes the conversion of ammonia and bicarbonate to carbamoyl phosphate in mitochondria. Non-small cell lung carcinoma (NSCLC) cells with oncogenic KRAS and loss of the tumor suppressor LKB1 express CPS1 and depend on this enzyme for growth. CPS1 maintains pyrimidine/purine balance in these cancer cells. Silencing CPS1 reduces the pyrimidine to purine ratio and stalls DNA synthesis, leading to DNA damage and cancer cell death. In addition, the tumor suppressor p53 represses the expression of urea cycle enzymes CPS1, OTC, and ARG1 and, therefore, causes the accumulation of ammonia, suppressing cancer growth. Furthermore, research studies suggest that hypermethylation-mediated downregulation of CPS1 expression may contribute to the progression of normal hepatocytes to hepatocellular carcinoma (HCC).

Concentration:1 mg/mL

Immunogen:Recombinant human CPS1 fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:1000-1:5000;IHC 1:50-1:100

Calculated MW:165 kDa

ObservedMW:165 kDa