Recombinant Cpn10 Monoclonal Antibody

SKU: AN301498L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:Cpn10

Target Synonym:CPN;HSP;HSPE;HSPE1;CPN10;EPF;GROES;HSP10;10 kDa chaperonin;10 kDa heat shock protein;10 kDa heat shock protein mitochondrial;CH10;Chaperonin 10;Chaperonin 10 homolog;cpn10 homolog;Early pregnancy factor;Early-pregnancy factor;GroES homolog;Heat shock 10kD protein 1 chaperonin 10;Heat shock 10kDa protein 1;Heat shock 10kDa protein 1 chaperonin 10;Heat shock protein family E (Hsp10) member;Heat-shock 10-kD protein;mitochondrial

Research Areas:Signal Transduction;Metabolism

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;IHC;IF

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A193

UNIProt ID:P61604

Accession:

Background:Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.

Concentration:1 mg/mL

Immunogen:Recombinant human Cpn10 fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:1000;IHC 1:200-1:1000;IF 1:50

Calculated MW:11 kDa

ObservedMW:11 kDa