Recombinant Cavia porcellus CTLA-4/CD152 Protein (His Tag)

SKU: PKSQ050093

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Immune Checkpoint Proteins

Abbreviation: CTLA-4;CD152

Target Synonym: CD152;CTLA-4;CTLA4;Cytotoxic T-lymphocyte protein 4;Cytotoxic T-lymphocyte-associated antigen 4

Research Areas:

Conjugation:

Target Species: Cavia porcellus

Expression Host: P.Pichia

Application: Cell culture

Fusion tag: C-His

UNIProt ID: H0VUB1

Accession: H0VUB1

Background: Cytotoxic Tlymphocyte 4(CTLA-4,CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily.CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.

Concentration:

Activity: Immobilized Mouse B7-1-Fc at 5 μg/ml (100 μl/well) can bind Cavia porcellus CTLA-4-His. The ED₅₀ of Recombinant Cavia porcellus CTLA-4-His is 4.29 ng/ml.

Sequence: Ala37-Asp161

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 16-35 kDa

ObservedMW: 18-40 kDa