Recombinant 2019-nCoV Guanine-N7_meth (N-6His)

SKU: EPT003

Size:50ug

Description:Recombinant 2019-nCoV Guanine-N7 methyltransferase is produced by our E.coli expression system and the target gene encoding Ala1-Gln527 is expressed with a 6His tag at the N-terminus.

Accession:YP_009725309.1

Molecular weight:62.9 KDa

Apparent molecular weight:60 KDa, reducing conditions

Other names:SARS-CoV 2 nsp14; SARS-CoV 2 ExoN; Guanine-N7 methyltransferase

Storage condition:Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Purity:Greater than 85% as determined by reducing SDS-PAGE.

Endotoxin:

Biological activity:

Redissolve:

Storage:Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Delivery condition:The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Background:The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.