RBL2(Phospho Ser952) Rabbit Polyclonal Antibody

SKU: BT-AP12244

Size:100μL

Background:Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and| in particular| that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2| leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation| associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.|miscellaneous:G0-restricted expression.|PTM:During G0 and early G1 phase of the cell cycle| phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.|Belongs to the retinoblastoma protein (RB) family.|subunit:Interacts with AATF. Interacts with SUV420H1 and SUV420H2 (By similarity). Component of the DREAM complex (also named LINC complex) at least composed of E2F4| E2F5| LIN9| LIN37| LIN52| LIN54| MYBL1| MYBL2| RBL1| RBL2| RBBP4| TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9| LIN37| LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with RINT1.|

Research_area:Cancer; Cell biology; Epigenetics and nuclear signaling

Target_protein:RBL2

applications:WB, ELISA

Reactivity:Human, Mouse, Rat

Clonality:Polyclonal

Clone ID:

Host:Rabbit

Isotype:IgG

Gene Symbol/ Name:RBL2

Immunogen:The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage:-20°C for 1 year

Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Molecular weight(Da):125290

UniProt accession:Human: Q08999; Mouse: Q64700; Rat: O55081

Synonyms:RBL2 ;Phospho Ser952; Retinoblastoma-like protein 2; 130 kDa retinoblastoma-associated protein; p130; Retinoblastoma-related protein 2; RBR-2; pRb2

GeneID:Human: 5934; Mouse: 19651; Rat: 81758